Inaugural Veterinary Drug Residue Course a Success!!

Our inaugural veterinary drug residue course "Methods of Determination for Drugs Residues in Aquaculture, Meat, and Poultry" was successfully completed in September!! Participants from Chile, Egypt, Iraq, Indonesia, Sudan and Kenya congregated at the JIFSAN-IFSTL facilities  where they learned about U.S. import regulations and the methods used to test veterinary drug residues.


The intensive eight day course consisted of lectures and practical laboratory work taught by U.S. FDA and USDA experts. Participants started with the regulatory side of the course with presentations from Margaret O'Keefe (USDA FSIS) about the regulations and compliance in meat and poultry, then  Barbara Montwill (FDA CFSAN) covered the regulations on aquaculture products. In a later lecture Dr. Clint Mitchell (FDA) then illustrated the drug approval process for veterinary drugs, highlighting the steps needed for each drug type. Talks then delved deeper into how regulations and analytical procedures overlapped with the presentations "Method Validation of Analytical Methods Drug Approval Process" by Dr. Philip Kijak (FDA CVM) and "Identification, Confirmation and Quantitative Analysis" by Dr. Hiranthi Jayasuriya (FDA CVM).

Introductory presentations on ultra high pressure chromatography (UHPLC)  and mass spectrometry (MS) by JIFSAN-IFSTL chemist Romina Heymann familiarized all participants with the basic principles of veterinary drug residue separation and detection. Afterwards hands-on sessions allowed participants to practice creating multi-residue multi-reaction monitoring methods for veterinary drugs on the Waters Xevo TQS system.

The laboratory work continued with six days of sample preparation following the principles of method validation introduced in the previous lectures. Samples were then tested by liquid chromatography with tandem mass spectrometry (LC-MS/MS), putting theory to practice. Participants learned about FDA nitrofuran testing, chloramphenicol in honey extractions, ELISA detection of chloramphenicol and the multi-residue method used by USDA regulatory labs. Important factors such as matrix effect were demonstrated on shrimp, tilapia, meat muscle, poultry and honey.

Joining us from Denver for the lab sessions was Dr. Sherri Turnipseed (FDA)  who gave a presentation entitled "Residue Analysis Method Development".  Dr. Turnipseed, who is well known for her expertise in residue analysis, was on hand during most of the week to answer questions from participants!

During the QuEChERS multi-residue testing, Dr. Steve Lehotay (USDA ARS) came to assist in the laboratory and to give a two part talk entitled "Method Development and Validation for the Monitoring of Multiple Veterinary Drug Residues in Animal Tissues". This in-depth presentation gave participants insight into the validation process and variables affecting the multi-residue procedure developed in his lab. Ms. Lucia Geis-Asteggiante, PhD student at the University of Maryland who has also worked at the USDA, joined Dr. Lehotay and assisted in the laboratory. In this laboratory session we were were able to test for differed veterinary drug residues such as thyreostats, phenicols, tetracyclines, fluoroquinolones, β-Agonist, coticosteriods,  β-lactams, nitroimidazoles, macrolides, anthelminitcs, NSAIDs, lincosamides, sulfonamides, and tranquilizers.

USDA scientist Kristi McEntire, who came all the way from the Athens (Georgia) USDA laboratory, was on hand to demonstrate the chloramphenicol ELISA testing. Also from the Athens laboratories, Chilton Ng gave an informative presentation entitled  "QA/QC: A Regulatory Perspective".

The final part of the training incorporated the participants processing their data and a final presentation of their results. In the presentation, recoveries, solvent calibration curves, matrix-matched calibration curves and the precision of their data were reported, then compared to the regulatory standards to decide whether their work passed the minimum requirements. The individual with the best recoveries won a prize, in this session Aldo Maddaleno arrived at the best recoveries from the team "Viva Chile!" (though Omar Qatr from the green team was a close second!).

The eight day course was a success, with each participant having gone from the regulatory process through four full sample preparations, development of MRM methods, LC-MS/MS detection, and data processing. Our 2013 class was an amazing group of individuals who worked hard for their certificates!! Great job!

Two participants extracting samples.


Groups of two or three participants extract tissue for veterinary drugs.

 Kristi McEntire from the USDA demonstrates how to wash ELISA plates.

Teaching assistant Lucia Geis-Asteggiante working hard!

Dr. Sherri Turnipseed from the FDA's Denver laboratories answering questions for participants.

Barbara Montwill presenting on the FDA's aquaculture regulations.

Dr. Steve Lehotay presenting on multi-residue testing.

Working in the laboratory.

Two participants finishing up an extraction.

Everyone letting loose in the laboratory after a week of hard work.

A participant working on finishing the lab session.

Dr. Steve Lehotay posing with the first group to complete the multi-residue laboratory.

Romina Heymann teaching how to create MRM methods on the LC-MS.

LC-MS/MS training

Waiting for the nitrogen evaporation step to finish.