This course will provide hands-on analysis of Cyclospora cayetanensis in fresh produce using Real-Time PCR. C. cayentanensis is a protozoan parasite responsible for waterborne and foodborne human diarrheal illness called cyclosporiasis. This disease occurs in developed and developing nations with certain seasonality. Due to the challenges with microscopic identification and culture techniques, the FDA has developed and validated an improved and streamlined molecular detection method for recovery and identification of C. cayetanensis from produce. This course will guide participants through the process for molecular identification following the methods outlined in the FDA's Bacteriological Analytical Manual (BAM) Chapter 19B.
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